Description:
Gene gun technology (particle bombardment) is a all-new gene-delivery technology, also a physical method, in which the genetic material or other substances attached to a high-speed microbomb are shot directly into the cells, so it is a late gene-transferred technology internationally. The pneumatic Gas gene gun can make a wide range of cell types instantly stable and highly-efficient transformation. The gene gun has a special rupture which generates a gas shock ware. The degree of Helium-pressure that drives the carrier film is determined by the different rupture films used in the process. Within a proper atmospheric pressure the different rupture films penetrate the DNA-coated microcariers into the target cells at the bottom of the bombardment chamber. The gene gun is applicable in gene-transformation of plants and animals, cell cultures, embryos, bacteria and small animals. It has fast, simple, safe and efficient features. The device purchased by Genetics, Neuroscience Research Institutes under , Fudan University, Zhejiang University, Shandong Agricultural University, and other relevant units .Articles concerming the product were published,such as:Zhejiang University gournal in the magazine 《Tea》2002.28(3),Plant transformation technology by using the gene gun and its application prospects in tea Cultivation; Fudan University Genetic institute published abroad, “Transformation and Functional Expression of the rFCA-RRM2 Gene in Rice” in《Plant Biology》.
Characteristics:
a unique circular bombardment transformation room, removable for easy cleansing and disinfection, low cost.
Standard Configuration: host, vaccuum pumps, breakage film, carrier film, stainless steel net, tungsten powder Experimental conditions In vitro, from the body, plants (in vivo)
- Target region (big)40cm2
- Pressure range 3-12MPa
- Target Type Animals: cell and organ culture Plants: The smaller of the whole plants, cell culture, explant, yeast, bacteria and other micro-organelles, chloroplast, mitochondria
- Voltage 220V/110V
- Gas Helium or nitrogen ( helium is more otimized)
- Metal Particle Φ0.6-1.6μm,(Common Φ1μm)