Description
The Sub Blot Mini and WAVE high intensity transfer systems combine the cooling capacity of wet submarine blotting units with the speed of semi-dry transfer systems. Both systems utilize plate electrodes to create a higher strength electric field and greater current density than conventional wire-electrode systems. This allows transfer of broad range molecular weight
proteins to be achieved typically within an hour in the Sub Blot Mini, and in 1-2 hours using the WAVE Sub Blot System. Features offered by the Sub Blot Mini and WAVE are as follows:
• Plate-electrodes 2cm apart in the Sub Blot Mini facilitate fast transfer of two stacked 8×8.5cm mini gels; and 4cm apart in WAVE Sub Blot system for transfer of one Maxi gel
• Multi-transfer capability in the WAVE also allows transfer of 4 omniPAGE Mini and 2 omniPAGE Mini Wide gels arranged side-by-side
• Two cooling options: a cool pack supplied with the Sub Blot Mini for environmentally friendly low-cost cooling; and active cooling in the Sub Blot WAVE by a detachable cooling
coil connected to a recirculating chiller (pg 81)
• Magnetic stirring bars fit conveniently beneath the cooling coil within the WAVE tank to maximise buffer circulation and heat dissipation, while preventing ion gradient formation; both tanks sit comfortably on most stirring plates
• Open design, rigid cassettes maximise gel-to-membrane compression for efficient current transfer; colour-coded to prevent polarity reversal
